黄芪注射液与葛根素注射液联用对2型糖尿病小鼠心脏损害的干预作用

摘要

Objective:To observe the effects of astragalus injection combined with puerarin injection on diabetic heart damage,and to lay a foundation for further study of its mechanism.Methods:Male KKAy mice(n=60)were fed with high fat diet to establish type 2 diabetes mellitus.Male C57BL/6J mice(n=30)with the same age were included into normal group.Mice with 12 weeks of age were randomly divided into treatment group and model group.The KKAy mice were injected with astragalus injection and puera-rin injection or the same amount of normal saline.After feeding at 8th,12th and 16th weeks,the general signs of mice were ob-served to detect the levels of the fasting blood glucose(FBG),serum triglyceride(TG),and total cholesterol(TCHO).The mor-phological myocardial tissue was observed,and TUNEL method was used to detect the level of apoptosis.Results:Astragalus injec-tion combined with puerarin injection could improve the symptoms of type 2 diabetes mellitus,slow activity,significant increase in urine output,lack of luster of body hair,body fat and other symptoms.After 6 weeks and 12 weeks treatment,it can significantly re-duce FBG,TG and TCHO(P<0.05),reduce myocardial cell apoptosis,improve myocardial fibrosis,but 16 weeks treatment was not significantly effective.Conclusion:The use of astragalus injection and puerarin injection can significantly improve the general state of type 2 diabetic mice,reduce FBG,TG and TCHO,inhibit myocardial cell apoptosis and reduce cardiac fibrosis.%目的:观察黄芪注射液与葛根素注射液合用对糖尿病心脏损害的干预作用.方法:选取60只KKAy雄性小鼠,以高脂饲料饲喂的建立2型糖尿病(T2DM)小鼠模型,将30只C57BL/6J雄性小鼠作为其正常对照组.用高脂饲料饲喂4周后(即12周龄时)把随机血糖≥13.9 mmol/L的小鼠随机分为观察组和模型组,分别注射黄芪注射液与葛根素注射液或等量生理盐水,给药8周、12周及16周后分批次处理小鼠,观察小鼠一般体征,检测其血液生化指标,观察心肌组织形态并运用TUNEL法检测细胞凋亡水平.结果:黄芪注射液与葛根素注射液联用能够改善2型糖尿病小鼠精神不佳,尿量增多,毛发欠光泽,肥胖等各种情况,对给药6周和给药12周糖尿病小鼠能显著降低其空腹血糖及血脂(P<0.05),减少心肌细胞凋亡,改善心肌组织纤维化,但是给药16周效果不明显.结论:黄芪注射液与葛根素注射液联合使用能够使2型糖尿病小鼠的一般状态得到显著改善,对模型小鼠的高血糖和高血脂有降低作用,可抑制心肌细胞凋亡并减轻心脏纤维化.