牙龈卟啉单胞菌感染对大鼠血管平滑肌细胞细胞间黏附分子-1表达的影响

摘要

Objective To observe the effects of Porphyromonas gingivalis (P. gingivalis) ATCC 33277 infection on ex-pression of intercellular adhesion molecule-1 (ICAM-1) in rat vascular smooth muscle cells(VSMC). Methods An infection model of rat VSMC invaded by P. gingivalis was established in vitro. The mRNA of ICAM-1 was measured through reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with the control group, an apparent and statistically significant increase in expression of ICAM-1 mRNA was observed after 8, 16, and 24 h in P. gingivals-infected rat VSMC (P<0.05). The expression reached its peak at 16 h. Statistically significant differences were observed in the 8 h group and in the other two experimental groups (P<0.05). Conclusion Infection of P. gingivals in rat VSMC can cause increased expres-sion of ICAM-1, which may have an important function in the progression of atherosclerosis.%目的：观察牙龈卟啉单胞菌ATCC33277感染对大鼠血管平滑肌细胞（VSMC）细胞间黏附分子-1（ICAM-1）表达的影响。方法建立体外牙龈卟啉单胞菌感染大鼠VSMC模型，逆转录-聚合酶链反应（RT-PCR）检测ICAM-1基因的表达。结果牙龈卟啉单胞菌感染VSMC8、16、24h后，ICAM-1表达明显增多，与空白对照组比较，差异有统计学意义（P<0.05）。感染16h达高峰，感染8h与感染16、24h比较，差异有统计学意义（P<0.05）。结论牙龈卟啉单胞菌感染可引起VSMCICAM-1高表达，这提示牙周致病菌可能参与血管壁的炎症反应，在动脉粥样硬化的发生、发展中有重要的意义。