黑龙江烟区烟草马铃薯Y病毒株系的分子鉴定

摘要

为明确黑龙江烟区烟草马铃薯Y病毒(Potato virus Y,PVY)株系种类及其分布状况,采用多重PCR技术及测序手段,对采集黑龙江省烟叶产区的107份疑似PVY样品进行了鉴定分析.结果表明:4个有代表性的PVY分离物HLJ-SH8、HLJ-SC2、HLJ-SC6和HLJ-MDJ4的基因组序列全长分别为9 698、9 702、9 702和9 698 bp,都包含一个由9 186 bp组成的开放读码框(ORF),编码一个由3 061个氨基酸组成的多聚蛋白.系统进化树分析结果表明这4个PVY分离物分别为PVYNTN、PVYN、PVYNW和PVYNTN-NW株系,其中PVYNTN-NW株系是侵染黑龙江烟草的优势株系.%To clarify the kinds and distribution states of tobacco Potato virus Y (PVY) strains in Heilongjiang tobacco planting areas, 107 suspected PVY isolates collected from Heilongjiang tobacco planting areas were identified with multiplex polymerase chain reaction (Multiplex-PCR) and sequencing techniques. The results indicated that the full length of genomes in four representative isolates (HLJ-SH8, HLJ-SC2, HLJ-SC6 and HLJ-MDJ4) were 9 698, 9 702, 9 702 and 9 698 bp, respectively. All of the four isolates contained an open reading frame (ORF) of 9 186 bp in length and encoded a polyprotein of 3 061 amino acids. Phylogenetic analysis indicated that the four isolates were PVYNTN, PVYN, PVYNW and PVYNTN-NW strains, respectively; among which, PVYNTN-NW strain was the dominant strain in Heilongjiang tobacco planting areas.