牡丹皮中多糖提取工艺的响应曲面法优化研究

摘要

为高效提取中药材牡丹皮中的多糖，利用响应曲面法优化回流提取牡丹皮多糖工艺。在单因素试验基础上，以提取温度、提取时间、水料比为响应因子，采用 Box －Behnken design 方法进行三因子三水平试验，以多糖得率为响应值，进行响应面分析。结果表明：提取温度与提取时间均显著影响牡丹皮多糖提取得率，三因子对多糖提取得率的影响作用大小依次为：提取温度﹥提取时间﹥水料比。牡丹皮多糖提取的最佳工艺条件为提取温度84℃、提取时间1．1 h、水料比31∶1，牡丹皮多糖提取得率预测值为3．732％，验证后得率为3．727％，与预测值基本吻合，相对误差为0．134％。本研究为牡丹皮的精深加工提供理论依据，同时为牡丹皮多糖构效关系的深入研究奠定基础。%To efficiently extract polysaccharides from moutan cortex,the response surface methodology was used to optimize the extraction technology.The Box -Behnken design method was used to conduct the 3 -factors and 3 -levels experiment on the basis of single -factor experiment for the responsive surface analysis. The extraction temperature,extraction time and ratio of water to material were the response factors.The yield of polysaccharides was the response value.The results showed that the yield of polysaccharides were signifi-cantly affected by extraction temperature and time.The effects of the 3 factors on polysaccharide yield were in order of extraction temperature,extraction time and ratio of water to material.The optimum extraction condi-tions were the extraction temperature as 84℃,extraction time of 1.1 hours and ratio of water to material as 31∶1.The predicted value of polysaccharide yield was 3.732% and the verified value was 3.727%.The veri-fied value was basically coincident with the predicted one with the relative error as 0.134%.This study could provide theoretical references for deep processing of moutan cortex and lay the foundation for studying on the structure -function relationship of polysaccharides from moutan cortex.