一株红树林真菌抗单纯疱疹病毒Ⅱ型的研究

摘要

目的:对分离自海南红树林真菌菌株PH0016的胞外多糖(exopolysaccharide,EPS)抗单纯疱疹病毒Ⅱ型(Herpes simplex virus type 2,HSV-2)活性进行研究.方法:收集纯化菌株PH0016产生的EPS,体外采用细胞病变观察EPS的细胞毒性和抗HSV-2作用.为观察EPS对HSV-2体内感染的治疗效果,小鼠颅内注射0.02ml滴度为100LD50/0.1ml-1的HSV-2,24小时后以不同浓度EPS灌胃,持续7天,14天后计算小鼠存活率和存活时间.菌株分类采用形态学观察和ITS序列分析.结果:EPS可抑制HSV-2病毒活性,病毒感染的细胞病变的半抑制浓度(Inhibited concentration,IC50)为313μ g/mL,SI为11.43.EPS 25mg·kg-1 ·d-1灌胃后,小鼠存活率为40.0％,存活时间为9.82±1.87天,相比模型组实验动物存活时间和存活率均有提高.菌株初步鉴定为淡紫色拟青霉.结论:从海南红树林分离一株淡紫色拟青霉,其产生的EPS具有抗HSV-2活性,值得进一步研究.%Objective:The antiviral activities of exopolysaccharide (EPS)from one kind of fungi strain PH0016 isolated from mangrove in Hainan province against HSV-2 was explored. Methods: The EPS of strain PH0016 was collected and purified. The toxicity and antiviral actions