急性胰腺炎患者外周血中Th17和Treg细胞及其细胞因子水平变化

摘要

ObjectiveTo study the changes of Th17 and Treg cells and the cytokines levels in the peripheral blood of patients with acute pancreatitis.MethodsNinety-two cases of acute pancreatitis treated in our hospital between August 2014 and August 2015 were selected and divided into three groups: MAP group (39 cases), MSAP group (29 cases),and SAP group (24 cases). Twenty-five healthy subjects were chosen as control group. The proportion of Treg and Th17 in peripheral blood from the four groups was detected by flow cytometry. mRNA of IL-17, IL-6, IL-10 and TGF-β in cells of peripheral blood was analyzed by real time PCR. Serum levels of IL-17, IL-6, IL-10 and TGF-β were detected by ELISA method.ResultsCompared with control group, the proportion of Treg cells and Treg/Th17 ratio in patients of MAP, MSAP and SAP groups significantly decreased (P<0.05), but the proportion of Th17 cells in patients of observation group was significantly increased (P<0.05). Compared with control group, IL-17 and IL-6 mRNA and protein levels in patients of MAP, MSAP and SAP groups were significantly increased (P<0.05), but IL-10 and TGF-β mRNA and protein levels in patients of observation group significantly decreased (P<0.05). ConclusionThere is imbalanced expression of Treg cells and Th17 cells and their cytokines in patients with acute pancreatitis, which is of clinical significance for study on the pathogenesis of acute pancreatitis.%目的：研究急性胰腺炎患者外周血中辅助性T细胞(Th17细胞)与调节性T细胞（regulatory cells，Treg细胞）及其细胞因子的水平变化。方法选取2014年8月—2015年8月来我院接受治疗的急性胰腺炎患者92例，其中轻度急性胰腺炎（mild acute pancreatitis，MAP）患者39例作为MAP组，中度急性胰腺炎（moderately severe acute pancreatitis，MSAP）患者29例作为MSAP组，重度急性胰腺炎（severe acute pancreatitis，SAP）患者24例作为SAP组，并以25名健康体检者作为对照组。采用流式细胞术分别检测4组外周血中Th17和Treg细胞比例，采用实时荧光定量PCR分析外周血白细胞中白介素17（IL-17）、白介素6（IL-6）、白介素10（IL-10）、转化生长因子-β（TGF-β）等因子mRNA水平，采用ELISA法检测血清中细胞因子IL-17、IL-6、IL-10、TGF-β等因子蛋白质水平。结果与对照组比较，MAP、MSAP和SAP组外周血Treg细胞比例、Treg/Th17比值明显下降，而Th17细胞比例明显升高（P＜0.05）。与对照组比较，MAP、MSAP和SAP组患者外周血细胞和血清中IL-10和TGF-β mRNA和蛋白质水平显著下降（P＜0.05)，而IL-17和IL-6 mRNA和蛋白质水平显著升高（P＜0.05）。结论急性胰腺炎患者外周血Treg细胞和Th17细胞比例以及相关细胞因子表达水平失衡，对进一步探讨急性胰腺炎发病机制具有重要意义。