湖州铁线莲多糖的分离纯化及体外抗肿瘤活性研究

摘要

[Objective]To isolate and purify the polysaccharide extracted from C.huchouensis Tamura and study their vitro antitumor activities.[Methods]Polysaccharide from C.huchouensis, named CHTP, was separated and purified by DEAE-Cellulose 52 ion-exchange chromatograph and Sephadex G-200 gel chromatograph.The anti-tumor activities of CHTP and its purified products were studied by MTT colorimetry.Three tumor cells such as HepG2, MGC8-03 and K562 were selected to detect their inhibitory effect.[Results]A polysaccharide fraction, named CHTPⅡ-1, was harvested after purified.When the concentration of CHTP was 250～1000 mg· L- 1 or the concentration of CHTPⅡ-1 was 125～500 mg· L 1, the inhibition rate of CHTP or CHTPⅡ-1 on HepG2, MGC8-03 and K562 cells increased with the increase of the polysaccharide concentration.There were significant differences of the OD value between the polysaccharide treatments and negative control except for MGC8-03 cells treated with 62.5 mg·L-1 CHTPⅡ-1 or K562 cells treated with 125mg· L-1 CHTP tested by MTT.[Conclusion]Both CHTP and CHTPⅡ-1 can inhibit the proliferation of the three tumor cells.Moreover, the inhibition rate is positively correlated to the amount of polysaccharides in test concentration.%[目的]分离纯化湖州铁线莲粗多糖(CHTP)并测定其体外杭肿瘤活性.[方法]采用DEAE-cellulose 52离子交换层析和Sephadex G-200凝胶过滤层析分离纯化CHTP;采用MTT法测定CHTP及经纯化后多糖(CHTPII-1)对体外培养的人肝癌细胞HepG2、人胃癌细胞MGC8-03和人白血病细胞K562等三种肿瘤细胞的抑制作用.[结果]经分离纯化获得多糖组分CHTPII-1;在CHTP浓度为250～1000mg·L(-1)或CHTPII-1浓度为125～500mg·L(-1)的检测范围内,CHTP和CHTPII-1对HepG2、MGC8-03和K562三种细胞的抑制率均随着多糖浓度的增大而增大.除了MGC8-03细胞62.5mg·L(-1)CHTPII(-1)处理组和K562细胞125mg·L(-1)CHTP处理组的MTT检测OD值与阴性对照组间无显著差异外,其余各组的MTT检测OD值均显著或极显著低于阴性对照组.[结论]CHTP和CHTPII(-1)对HepG2、MGC8-03和K562等三种肿瘤细胞均有较强的抑制作用,且其抑制率与多糖浓度呈良好的量效关系.