[Objective] Preliminarily clear the effective substance of Fuzi in treatment of AA by comparing the NO, TNF-α and toe volume in AA rats affected by diester alkaloids,monoester alkaloids and non-ester alkaloids. [Methods]Male wistar rats were randomly divided into comparing group and model group. FCA was given in the left rear toe of rat in model group(once a day,0.1mL each). After 2 weeks ’injection, model rats were randomly divided into model group, diester alkaloids group, monoester alkaloids group and non-ester alkaloids group. Rats in comparing group and model group were given physiological saline and others were given corresponding alkaloids. Detect the NO,TNF-αand toe volume after 2 weeks. [Results]Compared with the model group, the weight gain of rats in other group was suppressed(P<0.05). Compared with the model group, the toe volume and the NO, TNF-αof rats in the alkaloids groups reduced significantly(P<0.01).The toe volume of rats in monoester alkaloids group reduced more obviously. The NO of rats in diester alkaloids group and non-ester alkaloids group reduced more significantly than the rats in monoester alkaloids group( P<0.05). [ Conclusion] The diester alkaloids, monoester alkaloids and non-ester alkaloids in Fuzi al have a significant effect on AA rats and the effect of the monoester alkaloids is the best.%[目的]明确附子中双酯型生物碱、单酯型生物碱及非酯型生物碱对佐剂性关节炎大鼠血清一氧化氮（NO）、肿瘤坏死因子（TNF-α）水平及足趾容积变化的影响，初步明确其治疗佐剂性关节炎的药效物质。[方法]雄性wistar大鼠，左后足趾皮内一次性注射弗氏完全佐剂（FCA）0.1mL/只造模，2W后，取造模成功的大鼠，随机分为模型组、单酯型生物碱组、双酯型生物碱组及非酯型生物碱组，各给药组灌胃给予相应生物碱药液，给药2W后，检测血清中NO及TNF-α含量，造模及给药期间同时记录体重及足趾容积变化情况。[结果]与正常组比较，模型组与各给药组大鼠体重增长均受到抑制（P<0.05）；与模型组比较，各给药组血清中NO、TNF-α含量亦显著性降低（P<0.01），单酯型组及非酯型组在降低NO作用上较双酯型明显（P<0.05）；且各给药组大鼠的足趾容积显著降低（P<0.01），其中单酯型组效果更为明显。[结论]附子中单酯型、双酯型及非酯型生物碱对大鼠佐剂性关节炎有良好的治疗作用，其中单酯型生物碱的治疗作用更为明显。
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