[Objective] To probe the relation between the content of diester type and monoester type diterpenoid alkaloids in total alkalois and their hydrolysis products and the genuineness of Radix aeoniti lateralis. [ Method] Using HPLC analysis method, the content of diester type and monoester type diterpenoid alkaloids in total alkalois ami their hydrolysis products of Radix aeoniti lateralis was detected. [ Result] The content of diester type diterpenoid alkaloids of total alkaloidals from Radix aeoniti lateralis in Jiangyou, Sichuan Province was higher than that of in Yunnan and Hubei Province. The content of aconitine, mesaconitine, and hypaconitine were 0. 081% , 0. 161 and 0.314, respectively. The content of diester type diterpenoid alkaloids of hydrolysis products of total alkaloidals of Radix aeoniti lateralis in Jiangyou, Sichuan Province was 0. 004% , and the monoester type diterpenoid alkaloids was 0. 146% . The contents of benzoylaconine, benzoylmesaconine, benzoyl-hypaconine were 0. 016% , 0. 080% and 0.050% , respectively. [ Conclusion] The content of diester type diterpenoid alkaloids of total alkaloids of Radix aeoniti lateralis in Jiangyou, Sichuan Province was high, and the content of diester type and monoester type diteqjenoid alkaloids of their hydrolysis product was up to specification of Pharmacopoeia of People's Republic of China in 2010.%[目的]探索生附子总生物碱及其水解产物中双酯型和单酯型二萜生物碱的含量与附子道地性的关系 [方法]采用HPLC分析方法,测定四川江油、云南和湖北等地生附子总生物碱及其水解产物中的乌头碱、中乌头碱、次乌头碱等双酯型二萜生物碱和苯甲酰乌头碱、苯甲酰中乌头碱、苯甲酰次乌头碱等单酯型二萜生物碱的含量.[结果]四川江油道地产地栽培的生附子总生物碱中双酯型二萜生物碱含量为0.556％,远高于云南和湖北附子,其中的乌头碱、中乌头碱和次乌头碱含量分别为0.081％、0.161％和0.314％；其总生物碱高温水解产物中的双酯型二萜生物碱含量为0.004％,单酯型二萜生物碱的含量为0.146％,其中苯甲酰乌头碱、苯甲酰中乌头碱、苯甲酰次乌头碱的含量分别为0.016％、0.080％和0.050％.[结论]四川江油生附子双酯型二萜生物碱的含量高,其总生物碱高温水解后双酯型二萜生物碱的含量低,单酯型二萜生物碱的含量高,符合2010版药典规定.
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