目的探讨磁性饲养笼低频电磁场对家兔缺血肢体新生血管形成的促进作用。方法缺血组和缺血磁场组各24只新西兰家兔，动脉粥样硬化组和正常对照组各6只。缺血组和缺血磁场组动脉粥样硬化造模第42天作髂总动脉结扎缺血肢体造模，缺血磁场组手术当日放入磁性饲养笼内进行12毫特斯拉（m T ）的低频电磁场实验，每12 h 1次，每次30 min ，第7天测量4组双侧后肢体趾端皮肤温度和股动脉血流量并计算差值，检测血清缺氧诱导因子-1a（HIF-1a）及小腿横纹肌组织血管内皮细胞生长因子（VEGF）、CD34表达密度，并用光学显微镜观察小腿横纹肌组织细胞形态学变化。动脉粥样硬化组和正常对照组不作缺血肢体造模和低频电磁场实验。结果缺血组术后第7天双侧后肢体趾端皮肤温度及股动脉血流量平均差值均明显大于缺血磁场组（P ＜0．01），血清HIF-1a测量值、小腿横纹肌组织VEGF及CD34表达密度均明显小于缺血磁场组（P＜0．01）。动脉粥样硬化组和正常对照组小腿横纹肌组织VEGF及CD34未表达。光学显微镜观察缺血组小腿横纹肌组织第7天出现横纹肌片状坏死伴钙化、单核细胞浸润及横纹肌萎缩，缺血磁场组横纹肌空泡样变性并伴灶状坏死及横纹肌萎缩，动脉粥样硬化组小腿横纹肌组织脂肪变性。结论磁性饲养笼低频电磁场能明显促进家兔血清 H IF-1a生成、缺血肢体VEGF及CD34表达和新生血管形成，改善缺血肢体供血。%Objective:To investigate the role of the magnetic cages′low frequency electromagnetic fields in promoting neovascularization on the rabbits′ ischemic limb .Methods Controlled studies was adopted . The ischemic group and the ischemia magnetic field group consisted of 24 New Zealand rabbits each ,while artherosclersis and normal control group with 6 each .Atherosclerosis modeling at first was made of ische-mic and ischemia magnetic field groups through 42 days and then ischemic limbs modeling was done on iliac artery ligation of rabbits .On the seventh day the temperature of Ischemic group was measured then the bi-lateral limb toe-side skin and arterial blood flow were calculated .Afterwards the serum hypoxia inducing factor-1a (HIF-1a) was detected ,after determining the expression density of VEGF and CD34 the rabbits Objective:To investigate the role of the magnetic cages′low frequency electromagnetic fields in promoting neovascularization on the rabbits′ ischemic limb .Methods Controlled studies was adopted . The ischemic group and the ischemia magnetic field group consisted of 24 New Zealand rabbits each ,while artherosclersis and normal control group with 6 each .Atherosclerosis modeling at first was made of ische-mic and ischemia magnetic field groups through 42 days and then ischemic limbs modeling was done on iliac artery ligation of rabbits .On the seventh day the temperature of Ischemic group was measured then the bi-lateral limb toe-side skin and arterial blood flow were calculated .Afterwards the serum hypoxia inducing factor-1a (HIF-1a) was detected ,after determining the expression density of VEGF and CD34 the rabbits were killed to obtain the ischemic leg striated muscle tissue .We observed changes of leg striated muscle tissue morphological with an optical microscope .Ischemia magnetic field group on the day of surgery was put into the magnetic breeding cage for 12 milli-Tesla (mT ) low-frequency electromagnetic field 30 minutes each 12 hours ,and the same indicators were checked on seventh day .The artherosclersis and normal con-trol group were not tested in the ischemic limbs modeling and experiment of low-frequency electromagnetic field ,while the detection indicators were same to the ischemic group .Results Artherosclersis group was 4 .6 times normal in blood total cholesterol and 1 .7 times in triglyceride .The rabbits′temperature of bilat-eral limb toe skin and femoral artery flow of the first 7d average difference between the ischemic group were significantly greater than the ischemia magnetic field group (P <0 .01) in inspection of q ,the serum HIF-1a measured values and expression density of VEGF and CD 34 in ischemic calf striated muscle ische-mic group were significantly lower than the ischemia magnetic field group (P<0 .01) in inspection of q ,In the artherosclersis and control group groups calf striated muscle of VEGF and CD34 did not express .Un-der an optical microscope ,the ischemic group calf striated muscle tissue appeared lamellar necrosis and cal-cification ,infiltration of mononuclear cells in gap associated with of striated muscle on seventh day ,the is-chemia magnetic field group striated muscle appeared only vacuolar degeneration and focal necrosis and muscular atrophy .In the artherosclersis group ,calf striated muscle tissues were full of lipoid degenera-tion .Conclusion Magnetic cages with low frequency electromagnetic fields can promote formation of HIF-1a in the serum and expression of VEGF and CD34 on ischemic limbs significantly and improve angiogene-sis and supply of the blood in ischemic limbs .
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