[目的]了解麻疯树扦插生根过程中插条体内吲哚乙酸氧化酶( IAAO)、过氧化氢酶(CAT)的变化规律.[方法]采集不同地方的麻疯树4类无性系插条进行大田扦插,在扦插成活过程中,分期采集不同类无性系插条并分段进行IAAO与CAT酶活性测定分析.[结果]在麻疯树扦插过程中,插条下部IAAO活性逐渐下降；在愈伤组织形成、根伸长期,插条CAT活性增加；枝条上、下部IAAO活性比中部高,而各段的CAT活性差异较小.在不同无性系之间,成活率较高或树龄较小的插条,其IAAO活性较低；对于CAT活性,则以树龄较小的无性系酶活性相对较高,而树龄较大的酶活性相对较低.[结论]在扦插生根过程中,不同地区插条的树龄、成熟度和成活率对其体内的IAAO与CAT均有一定影响,研究结果可为选择合适的插条提供参考.%[Objective]The present experiment was conducted to investigate the changes in activities of IAAO and CAT in cuttings of Jatropha curcas L. [Method]Four cutting types were collected from upper, middle and down parts of Jatropha curcas L. Young and adult trees planted in Panzhihua city of Sichuan and Dashao of Yunnan Province, and were planted in field of Wanbao Village of Yuanmou, Yunnan. The activities of IAAO and CAT in cutting were detected during cuttage process. [Result]During cuttage process of Jatropha curcas L. Cuttings, the IAAO activity in bottom cuttings decreased, and the CAT activity increased during callus formation and root elongation stage. The IAAO activity in the top and bottom sections of cutting was higher than that of the middle section, while there was no significant difference in CAT activity in different cutting sections. In different clones, the IAAO activity of clones with higher survival rate or collected from young tree was lower, while the CAT activity of younger clones was higher than older clones. [Conclusion]During cuttage process of Jatropha curcas L. Cuttings, different age, maturity and survival rate of tree have significant effects on the activities of IAAO and CAT. The results obtained in the present studies will provide basis for selecting suitable cuttings.
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