金欣口服液对感染RSV大鼠血清IL-12表达水平的影响

摘要

目的 现察金欣口服液对感染呼吸道合胞病毒(RSV)大鼠血清IL-12表达水平的影响,以寻找更有效的抗RSV感染方式.方法 将96只SD大鼠随机分为12组,分别为空白对照组,模型组,金欣高、中、低灌胃组,金欣高、中、低灌肠组,利巴韦林灌肠、灌胃组,金欣预先灌胃、灌肠组.用双抗体夹心酶联免疫吸附法(ELISA)测定各组SD大鼠血清IL-12的水平.结果 与空白对照组比较,模型组感染RSV后血清IL-12水平明显下降(P＜0.01);金欣组的高剂量灌胃组、中剂量灌胃组、中剂量灌肠组及预先灌胃组的IL-12水平较模型组显著升高(P＜0.01),且与利巴韦林组有明显差异(P＜0.01).灌胃组中预先给药的IL-12含量比其他给药组相比显著增加(P＜0.01).与灌肠给药相比,灌胃给药的预先组和金欣高剂量组的IL-12较高(P＜0.01).结论 金欣口服液能提高血清IL-12的表达,且以灌胃方式提前给药能够更有效地抑制大鼠感染RSV.%OBJECTIVE To observe Jinxin Oral Liquid's effect on the expression of serum IL-12 of rats infected with respiratory syncytia virus (RSV) to seek more effective ways of anti-RSV infection. METHODS 96 SD rats were divided into 12 groups randomly and equally: control group, model group, higher, middle, lower dosages of Jinxin groups by intragastric administration (ig) (HIG, MIG, LIG), higher, middle, lower dosages of Jinxin groups by retention enema (HEG, MEG, LEG), ribavirin intragastric and enema group (RIG, REG), pre-Jinxin intragastric and enema group(PIG, PEG). Serum levels of IL-12 were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) of each group. RESULTS Compared with control group, IL-12 serum levels were significantly decreased in model group (P＜0.01); IL -12 levels in HIG, MIG, MEG and PIG were higher than those in model group significantly (P＜0.01), and were different from that of ribavirin group statistically (P＜0.01). IL-12 of PIG increased much more than other group by ig (P＜0.01).Compared with retention enema, IL-12 levels were higher in PIG and HIG (P＜0.01).CONCLUSION Jinxin Oral Liquid can increase serum IL-12 expression, and it is among effective way to inhibit RSV infection by intragastric administration in advance.