酶联合消化法培养小鼠主动脉平滑肌原代细胞

摘要

目的：建立一种适用于体外有效分离培养小鼠主动脉血管平滑肌细胞的技术方法。方法：分离主动脉，用优化的酶联合消化法获取主动脉平滑肌细胞，用形态学法、免疫细胞化学法鉴定。结果：该方法所分离的主动脉平滑肌细胞生长旺盛，细胞活性好，7～9d后细胞总量可达到5×105个/瓶，细胞纯度可达85％以上，细胞呈长梭形、放射状、束状生长，平行排列呈峰谷状，免疫荧光显示胞浆内α-SM A蛋白表达阳性。结论：该酶联合消化法可在体外短时间内获取数量可观、高纯度的主动脉平滑肌细胞。%Objective :To establish a technical method that was effectively isolated and cultured mouse aortic vascular smooth muscle cells in vitro .Methods :Separated aortic ,harvested aortic smooth muscle cells with optimization method , identified using morphological method and immunocytochemistry method .Results:Vascular smooth muscle cells were obtained by this method could grow fast and well ,reaching 5 × 105 after five or six days ,immunofluorescence staining showed that the purity was more than 85% ,intracytoplasmic protein expression of α-SMA-positive ,the cell morpholo-gy were long spindle ,radial growth ,wispy growth ,and showed the typical “peak and valley” morphological appear-ance .Conclusion:This new method could obtain considerable number of high-purity aortic smooth muscle cells in vitro .