芦笋花药培养愈伤诱导条件优化与植株再生

摘要

In order to establish a better system of Asparagus anther culture technology, a plant of the first filial generation ' Apollo' which had a better character in the process of growth was selected. The results showed that the best callus and shoots inducted conditions were buds whose lengths were 1 - 2 mm, storage at 4 ℃ for 5 days and a MS culture medium containing 2. 0 mg/L 6-BA, 1. 0 mg/L NAA; the best subculture medium was MS+0. 5 mg/L 6-BA+0. 2 mg/L NAA. Plant regenerations from callus were ploidy identified.%以栽培表现较好杂交一代品种‘Apollo’为试材,研究了芦笋花药培养技术,建立了一套较完善的芦笋花药培养技术体系.结果表明:1～2 mm的花蕾、4℃低温预处理5d、MS+2.0 mg/L 6-BA+1.0 mg/L NAA为愈伤组织和芽诱导的最佳条件；最适继代增殖条件为:MS+0.5 mg/L 6-BA+0.2 mg/L NAA.并对继代增殖组培苗进行了初步的倍性鉴定.