超声波辅助提取葡萄酒泥酵母超氧化物歧化酶工艺优化

摘要

Taking waste wine yeast as the research obj ect,the superoxide dismutase(SOD)was extrac-ted from it by ultrasonic wave.Four extraction parameters including material-liquid ratio,ultrasonic power, ultrasonic time and thermal denaturation temperature were studied by single factor test,and the optimum process parameters were determined by orthogonal test.The results showed that the optimal material-liquid ratio was 1∶2.5,ultrasonic power was 400 W,ultrasonic time was 16 min,thermal denaturation temperature was 45 ℃,under these conditions,the specific activities of SOD was 169.32 U/mg.The process was sim-ple,inexpensive and the specific activities of SOD was high.%以葡萄酒泥酵母为试验材料,采用超声波辅助法分离提取超氧化物歧化酶(SOD).通过单因素试验,研究了料液比、超声波功率、超声波时间和热变性温度对 SOD 比活力的影响,并采用正交试验确定了超声波法分离提取 SOD的最佳工艺参数.结果表明：葡萄酒泥酵母 SOD提取的最佳工艺参数为料液比1∶2.5、超声功率400 W、超声作用时间16 min、热变性温度45℃,在此条件下,分离提取得到的 SOD比活力最高为169.32 U/mg.此方法操作简单,成本低,所得 SOD比活力较高.