GoldeneyeTM DNA身份鉴定系统25A试剂盒的法医学验证

摘要

目的：对GoldeneyeTM DNA身份鉴定系统25A试剂盒的技术性能进行测试及评估。方法依据规定的方法对该试剂盒的准确性、灵敏度、一致性、均衡性、稳定性和混合样本等方面进行检验，并应用于部分人员血样和常见案件检材的检验。结果各等位基因间均衡性≥42%，阳性DNA样本分型准确，0．125 ng的阳性对照样本可检出完整分型。结论 GoldeneyeTM DNA身份鉴定系统25A试剂盒可以用于法庭科学的DNA数据库和部分案件的DNA检验。%Objective To test and estim ate the forensic application of G oldeneyeTM DNA ID 25A K it. Methods The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and m ixed sam ples through m easured blood sam ples and other sam ples in routine casework. Results The peak height balance of the different loci was ≥42% . The genotyping results of the positive control DNA was accurate. The com plete STR genotyping result could be obtained from 0.125 ng positive control DNA . Conclusion G oldeneyeTM DNA ID 25A K it is suitable for crim inal cases and DNA database in forensic practice.