高效液相-阳离子交换色谱法测定藏药安神丸中槟榔碱的含量

摘要

目的 建立高效液相-阳离子交换色谱法测定藏药安神丸中槟榔碱含量的方法.方法 样品用乙醚溶液和碳酸盐缓冲液提取后,用SepaxPolysulfonix-SCX (250 mm×4.6 mm,5 μm)强阳离子交换树脂色谱柱分离,以乙腈-0.2％磷酸溶液(pH 3.8,10∶90,V∶V)作为流动相进行洗脱,0.5 mL/min流速,用紫外检测器于215nm波长处进行测定.结果 氢溴酸槟榔碱在7.028～210.840 μg/mL范围内线性关系良好(r2=0.9998),回归方程为Y=44.5058X-56.3314,加标回收率为95.67％～97.41％,相对标准偏差为1.07％～1.80％(n=3).结论 此方法具有良好的精密度、稳定性及回收率,可用于安神丸中槟榔碱成分的测定.%Objective To establish a method for determination of the arecoline content in Tibetan medicine Anshen pills by high performance liquid chromatography-cation exchange chromatography.Methods The samples were extracted with a mixture of ether solution and buffer bicarbonate,separated by Sepax Polysulfonix-SCX (250 mm×4.6 mm,5 μm) with acetonitrile-0.2％ phosphoric acid solution (pH3.8,10∶90,V∶V) as mobile phase for elution,and detected by ultraviolet detector in 215 nm.Results Arecoline had good linearity in the range of 7.028～210.840 μg/mL (Y=44.5058X-56.3314,r2=0.9998),the average recoveries ranged from 95.67％～97.41％,and the relative standard deviations was 1.07％～1.80％ (n=3).Conclusion This method has good precision,stability and recovery rate,which is suitable for determination of arecoline quantity in Anshen pills.