This study was to purposed to explore the methylation status changes of IEX-1 gene promoler CpG island and its relevance with occurrence of hematologic malignancies.The methylation starus of IEX-1 gene promoter CpG island in 9 NB4, HL-60 U937, Raji, CA46, Jurkat, K562, CEM and Molt4 hematologic malignant cell lines was detected by using methylation-specific PCR, the methylation starus of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells treated by M.sssI enzyme and the methylation status of IEX-1 gene promoter CpG island in normal peripheral blood mononuclear cells untreated were used as positive and negative controls respectively.The results showed that the hypermethylation of IEX-1 gene promoter CpG islands was detected in NB4, Molt4 and Raji cell lines, as well as in normal peripheral blood mononcelear cells treated by M.sssl enzyme; the partial methylation status was found in CA46, CEM, U937, K562, HL-60 and Jurkat cell lines; the unmethylation status was observed in untreated normal peripheral blood mononcelear cells.It is concluded that the changes of methylation status of gene IEX-1 promoter CpG island correlates with hematologic manignancies to a certain extent.%本研究探讨基因IEX-1启动子区CpG岛甲基化状态的改变及其与恶性血液病发生的相关性.应用MSP的方法检测9种恶性血液病细胞系中IEX-1基因启动子区CpG岛甲基化状态,并把经M.sssI酶处理过的正常人外周血单个核细胞中IEX-1 CpG岛甲基化状态作为阳性对照,把正常人外周血单个核细胞中IEX-1 CpG岛甲基化状态作为阴性对照.结果显示:在NB4、Molt4.、Raji细胞系中IEX-1基因启动子区CpG岛呈高甲基化状态;在CA46、CEM、U937、K562、HL-60、Jurkat细胞系中IEX-1基因启动子区CpG岛呈部分甲基化状态;在正常人外周血单个核细胞中IEX-1基因启动子区CpG岛呈非甲基化状态;经M.sssI酶处理过的正常人外周血单个核细胞中IEX-1基因启动子CpG岛呈高甲基化状态.结论:IEX-1基因启动子区CpG岛甲基化状态的改变与恶性血液病有一定的相关性.
展开▼
