目的 观察多次食管酸灌注对哮喘小鼠气道瞬时感受器电位香草酸受体-1(Transient receptor potential vanilloid-1,TRPV1)受体的影响.方法 60只雄性BALB/c小鼠随机分成6组(正常组、灌盐组、灌酸组、哮喘组、哮喘灌盐组、哮喘灌酸组),用OVA致敏和激发的方法建立哮喘模型,用食管下段盐酸灌注建立胃食管反流(gastroesophageal reflux,GER)模型,末次抗原激发后24小时处死小鼠,HE染色观察小鼠食道及肺组织病理改变;免疫组化及荧光定量PCR检测TRPV1受体蛋白及mRNA在小鼠肺组织中的表达及分布情况.结果 与正常组与灌盐组比较,灌酸组、哮喘组小鼠肺部均表现出明显的炎症反应,气道上TRPV1表达上调,尤其是哮喘灌酸组表现明显,与其他组相比有显著差异性(P<0.001).结论 多次食管酸灌注可明显上调哮喘小鼠TRPV1受体的表达,推测TRPV1受体的激活可能参与了胃食管反流引起哮喘发生发展的发病机制.%Objective To observe the influence of TRPV1 receptors in airway by repeating esophageal acid infusion in mice with asthma. Methods A total of 60 male BALB/c mice were randomly divided into six groups ( the control group, the salt group, the HCl group, the asthma group, the asthma and salt group, and the asthma and HCl group) . Asthma model was established by intraperitoneal injection and atomization chicken ovalbumin ( ovalbu-min, OVA) . Mice were sacrificed in 24 hours after the last antigen stimulation. After centrifugation, HE staining was used to observe histopathological changes of lung tissues and esophagus. Immunohistochemistry was performed for detecting the expression and distribution of protein, while quantitative PCR was done for detecting mRNA of TRPV1 receptor in mouse lung tissue. Results Compared with the normal group and the salt group, the asthma and HCl group showed significant inflammation in lung and TRPV1 mRNA expression level was up-regulated, especially in the asthma and HCl group ( P<0. 001 ) . Conclusion TRPV1 mRNA expression is significantly up-regulated in mice with asthma by repeating esophageal acid infusion. Presumably, the activation of TRPV1 receptors in lung and esoph-agus plays a role in the development of asthma caused with gastroesophageal reflux.
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