食管酸灌注对哮喘小鼠气道炎症及BRP-39浓度的影响

摘要

目的 观察食管酸灌注对哮喘小鼠气道炎症的影响及BRP-39浓度与哮喘小鼠气道炎症的关系.方法 60只雄性BALB/c小鼠随机分成6组(正常组、灌盐组、灌酸组、哮喘组、哮喘灌盐组、哮喘灌酸组),用OVA致敏和激发的方法建立哮喘模型,用食管下段盐酸灌注建立胃食管反流(gastroesophageal reflux,GER)模型,末次抗原激发后24小时处死小鼠,ELISA法检测回收的BALF及血清中的BRP-39;BALF细胞沉渣涂片后染色计数细胞总数及分类计数. 结果 (1)单纯哮喘组及哮喘灌盐、哮喘灌酸组小鼠BALF中细胞总数及嗜酸性粒细胞、中性粒细胞、淋巴细胞的细胞分类数均较对照组高,以嗜酸性粒细胞增高最为明显,具有统计学意义(P<0.05);灌酸组小鼠BALF中细胞总数及中性粒细胞、淋巴细胞的细胞分类数均较对照组高,以中性粒细胞增高最为明显,具有统计学差异(P<0.05);正常组与灌盐组小鼠,哮喘灌盐组与哮喘灌酸组小鼠无统计学差异.(2)与对照组比较,除灌盐组外,其余各组特别是哮喘灌酸组,BALF及血清中BRP-39的浓度均明显升高,且与BALF中的细胞总数及中性粒细胞、嗜酸性粒细胞呈正相关(P<0.05~P<0.01) 结论 多次食管酸灌注可引起气道内炎症反应,伴有血清及BALF中的BRP-39浓度明显升高,可能与GER诱发或加重哮喘有关.%Objective To observe the influence of airway inflammation by repeating esophageal acid infusion and the relationship between BRP-39 levels and airway inflammation in mice with asthma.Methods A total of 60 male BALB/c mice were randomly divided into six groups (the control group, the salt group, the HCl group, the asthma group, the asthma and salt group, the asthma and HCl group).Asthma model was established by intraperitoneal injection and atomization chicken ovalbumin (OVA).HCL was used to do lower esophageal perfusion to establish GER model.Mice were sacrificed in 24 hours after the last antigen stimulation.The BRP-39 levels in serum and BALF of the mice were measured by enzymelinked immunosorbent assay (ELISA).The total and classification cell numbers in BALF were recorded.Results (1) The total cells, eosinophils, neutrophils and lymphocytes of BALF in the asthma model group (including the asthma group, the asthma and NS group, and the asthma and HCL group) were higher than those in the control group, especially for eosinophils (P<0.05-P<0.01).Compared with the control group, the total cells, neutrophils and lymphocytes in BALF of the HCL group were higher, especially for neutrophils (P<0.05-P<0.01).There was no statistically difference in Eosinophilic cells between the HCL group and the control group.(2) Compared with the normal group, in addition to the salt group, BRP-39 concentrations in BALF fluid and blood increased in the other groups (P<0.05), especially in the asthma and HCL group.And there was a positive correlation between BRP-39 levels and the number of BALF, neutrophils, eosinophils (P<0.05-P<0.01).Conclusion Repeated?esophageal acid?perfusion?can cause?inflammation in lung, and BRP-39 levels in serum and BALF increase.GER can cause or aggravate asthma, which may be associated with BRP-39 levels.