[ Objective ] The study aimed to screen out Lactobacillus plantarum possessing higher inhibitory activity against Penicillium roqueforti and enhance its antifungal activity. [ Method] The activated L. plantarum IMAU10116 was innoculated MRS liquid medium for culturing and double-layer plate point inoculation was used to evaluate its activity against P. roqueforti. [ Result] The single-factor experiments showed that when glucose was used as carbon source, the inhibitory effect of L. plantarum 1MAUl0116 against P. roqueforti was best; when malt extract powder was used as nitrogen source, its inhibitory effect was best; the optimum growth factor for antifungal lactobacillus producing antifungal substance was powder of corn steep liquor. The optimum medium composition of L. plantarum IMAU10116 was 20 g/L glucose + 20 g/L malt extract powder + 7 g/L powder of corn steep liquor + quantum sufficit mineral salt. The diameter of inhibition zone of L. plantarum IMAU10116 cultured with optimized medium against P. roqueforti was 18.6 mm and that of L. plantarum IMAU10116 cultured with MRS medium was 15.0 mm. [Conclusion] L. plantarum IMAU10116 had very good inhibition activity against P. roquefoni and it was hopeful to get better application in food and forage additive domain.%[目的]筛选对娄地青霉有较强抑菌活性的植物乳杆菌并提高其抗真菌活性.[方法]将活化后的植物乳杆菌IMAU10116接种到MRS液体培养基中培养,采用双层平板点接法评价其杭娄地青霉菌的活性.[结果]单因素试验表明,以葡萄糖为碳源时,植物乳杆菌IMAU10116对娄地青霉的抑制效果最好;植物乳杆菌以麦芽浸粉为氮源时,抑菌效果最好;抗真菌乳酸菌产生杭菌物质的最佳生长因子为玉米浆粉植物乳杆菌IMAU10116最佳培养基组成为:葡萄糖20 g/L+麦芽浸粉20 g/L+玉米浆粉7 g/L+适量无机盐.用优化培养基培养的植物乳杆菌IMAU10116抑制娄地青霉菌产生的抑菌圈为18.6 mm,用MRS培养基所产生的抑菌圈为15.0 mm.[结论]植物乳杆菌IMAU10116对娄地青霉有很好的抑制作用,有望在食品及饲料添加剂领域获得较好的应用.
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