同时高产甘露聚糖肽与乳酶生发酵条件的研究

摘要

[目的]探索同时高产甘露聚糖肽和乳酶生的最佳发酵条件.[方法]以甲型溶血性链球菌H1S-33号为菌株,进行液体发酵培养,在各个时间段分别取样测定活菌数及甘露聚糖肽含量,确定最佳培养条件.[结果]在液体发酵过程中,28 h发酵液中甘露聚糖肽含量最高为69.2 μg/ml,活菌数最多为2.38×1016个/ml.[结论]同时高产甘露聚糖肽与乳酶生发酵条件为:培养基由牛肉膏、蛋白胨、酵母骨、氯化钠组成,发酵温度37℃,最佳发酵时间28 h.%[ Objective ] The aim was to explore the fermentation condition of high yield mannatide and lactasin simultaneously. [ Method ] In order to obtain the best culture conditions, in the period of liquid fermentation culture, using α-hemalytic streptococci 33 as strains, all living bacteria number and mannatide content were determined respectively. [ Result] In the liquid fermentation process, the highest content of mannatide was 69.2 μg/ml, the maximum number of living bacteria was 2.38 × 1016/ml. [ Conclusion] The best fermentation conditions were made from beef cream medium, peptone, yeast creams, sodium chloride composition, at temperature 37 ℃ with 28 h.