显微镜观察药物敏感性技术快速检测痰标本中结核分枝杆菌的研究

摘要

Objective To study the effect of microscopic observation drug susceptibility assay (MODS)for testing Mycobacteri-um tuberculosis in sputum ,and to evaluate its clinical value .Methods A total of 150 sputum samples were collected from patients with pulmonary tuberculosis ,and Mycobacterium tuberculosis were detected by Lowenstein-Jensen (L-J) method and MODS ,and compared the results of the two methods .Results The concordance rates of the test results of sputum sample between MODS and L-J was 77 .8% .The sensitivity and specificity of MODS were 93 .3% and 70 .0% ,positive predictive value (PPV ) was 60 .8% , negative predictive value (NPV) was 95 .4% ,as well as accuracy by MODS was 77 .8% .Conclusion MODS assay could be used as a rapid tuberculosis detection method ,with rapid ,sensitive ,simple ,and other advantages .%目的：探讨显微镜观察药物敏感性技术（MODS）在痰液标本中结核分枝杆菌（M TB）的检测效果，以评价其临床应用价值。方法收集150份患者临床痰标本，应用 MODS 技术、罗氏培养法进行 M TB 的检测，将两法的结果进行对比分析。结果 MODS技术与罗氏培养法结果符合率为77．8％，MODS 技术检测痰标本中 M TB 的敏感性为93．3％，特异性为70．0％，阳性预测值（PPV）为60．8％，阴性预测值（NPV）为95．4％，正确性为77．8％。结论 MODS 技术检测痰标本中 M TB 具有快速、灵敏、简便等优点，可作为结核病细菌学快速检测的新方法之一。