Objective: To investigate whether apelin-13 has angiogenesis effects in myocardial micro-vascular endothelial cells (MVECs). Methods: Rat MVECs were cultured in vitro. The cells were treated with apelin-13 at different concentrations. MTT assay was used to determine the proliferation ability. Migration ability was measured using cell scratches and a modified boyden chamber. The tube formation ability of MVECs was tested by Matrigel. The phosphorylation of AMPK and Akt which may be induced by apelin-13 was measured with Western blot. Results: Apelin enhanced the proliferation, migration and tube-like structure formation of MVECs in vitro, which was concentration dependent. Furthermore, apelin-13 signicantly up-regulated the phosphorylation levels of both Akt and AMPK. Conclusion: Apelin has angiogenesis effect in MVECs, which may be related to the upgrading of Akt/AMPK phosphorylation.%目的:探讨apelin-13对大鼠心肌微血管内皮细胞(MVECs)的生物学效应及其可能的信号通路. 方法:体外培养大鼠的心肌微血管内皮细胞.给予不同浓度apelin-13,采用MTT法观察细胞增殖能力、划痕损伤修复和Transwell方法观察细胞迁移能力、体外管腔样结构形成方法观察细胞管腔成能力.进一步采用蛋白印迹法观察apelin-13对Akt和AMPK分子蛋白的磷酸化水平的影响. 结果:Apelin-13能增加大鼠微血管内皮细胞的增殖、迁移和体外管腔形成能力,且呈剂量依赖性在200 nmol/L浓度时促血管生成能力最佳.Apelin-13能上调细胞的Akt和AMPK磷酸化程度. 结论:Apelin-13可促进MVECs的血管生成,可能与上调Akt和AMPK的磷酸化相关.
展开▼
