通过基因工程手段增加厌氧氨氧化菌亚硝酸盐还原酶(nitrite reductase, nirS)的表达量,运用质粒载体pGEM-T克隆nirS基因。琼脂糖凝胶电泳检测显示, nirS基因重组工程菌在440 bp处有明显的目的条带； nirS基因重组工程菌扩大培养7~8h后即达到生长曲线稳定期,引入外加氮源后,菌体生长情况更优。通过不同菌液投加量以及处理不同初始浓度的亚硝酸钠溶液,检测nirS基因重组工程菌的性能。结果表明,当nirS基因重组工程菌投加30 mL(细菌数为2.3×107个∕mL),亚硝酸盐初始质量浓度为40 mg∕L时,亚硝酸盐去除率达到90%以上。nirS基因重组工程菌可适用于亚硝酸盐废水的处理。%In order to improve the expression quantity of nitrite reductase (nirS) in ANAMMOX bacteria through bioengineering means, nirS gene was cloned using the plasmid vector pGEM-T. A target band of 440 bp PCR products from the recombinant genetic engineering bacter was observed by agarose gel electrophoresis. The nirS recombinant genetic engineering bacteria reached stationary phase after 7-8 hours incubation, the addition of nitrogen source was advantageous to the growth of bacteria significantly. The performance of nirS recombinant genetic engineering bacteria was tested by adding different dosages of bacteria and treating sodium nitrite solu‐tion with different initial concentrations. The results showed that, when 30 mL of nirS recombinant genetic engi‐neering bacteria(2.3 × 107 cells/mL) inoculates was added to the solution with 40 mg/L of initial mass concentra‐tion of nitrite, the removal rate of nitrite reached above 90%. It was indicated that nirS recombinant genetic en‐gineering bacteria could be applied for nitrite-containing wastewater treatment in the future.
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