不同基本培养基和激素对籼稻成熟胚愈伤组织诱导的影响

摘要

Effects of four basal medium (MS,N6,NB and NMB) and two kinds of phytokinin (KT.6-BA) on callus induction from mature embryos of four indica rice varieties (Chuanxiang29B, Zhong9B, Jin23B, Ⅱ-32B) were investigated. The results showed that the most efficient basal medium was NMB as the average callus induction rates ranged from 77.00% to 96.00%. The order of average rate from high to low was Chuanxiang29B, Zhong9B, Ⅱ-32B, Jin23B. When 3 mg/L 2, 4-D was applied with 0.1 mg/L KT or 0.4 mg/L 6-BA, the induction rate of Chuangxiang29B was the highest. For Zhong9B, the combination of 3 mg/L 2, 4-D with 0.1 mg/L KT or 0.1 mg/L 6-BA was optimum. For Ⅱ -32B, the combination of 3 mg/L 2, 4-D with 0.6 mg/L KT or 0.1 mg/L 6-BA was optimum. However, the callus formation of Jin23B would be inhibited by adding either 6-BA or KT.%以川香29B、中9B、金23B和Ⅱ-32B 4种籼稻的成熟胚为外植体,研究MS、N6、NB和NMB 4种基本培养基,2,4-D与KT或6-BA联合使用对籼稻愈伤组织诱导的影响.结果表明,4种籼稻在NMB基本培养基中愈伤组织的诱导率最高,平均诱导率为77.00％～96.00％,其中在NMB中的诱导效果最好,在4种不同基本培养基中愈伤组织总平均诱导率的高低顺序依次为川香29B、中9B、Ⅱ-32B、金23B.当3.0mg/L 2,4-D与不同浓度的KT或6-BA联合使用对,KT浓度为0.1 mg/L对,川香29B和中9B愈伤组织诱导率高；对于Ⅱ-32B,KT浓度为0.6 mg/L愈伤组织诱导率较高；6-BA浓度为0.4 mg/L时,川香29B愈伤组织诱导率最高；对于中9B和Ⅱ-32B,添加0.1 mg/L的6-BA最有利于愈伤组织的诱导；而对于金23B,无论是添加KT或6-BA都会抑制愈伤组织的诱导.