急性白血病患者血清及脑脊液中基质细胞衍生因子-1α的检测意义

摘要

目的 探讨急性白血病(AL)患者血清及脑脊液(CSF)基质细胞衍生因子-1α(SDF-1α)的表达水平及临床意义.方法 采用酶联免疫吸附法(ELISA)分别检测初诊、未治和经标准方案化疗2个疗程后的56例AL患者以及10例同期颅脑外伤患者(对照组)的血清及CSF中SDF-1α的水平.结果 初诊未治时,急性淋巴细胞白血病(ALL)组、急性髓细胞白血病(AML)组、中枢神经系统白血病(CNSL)组和非中枢神经系统白血病(N-CNSL)组血清、CSF中SDF-1α浓度均明显高于对照组(P<0.01),且ALL组明显高于AML组(P<0.01),CNSL组明显高于N-CNSL组(P<0.01).经标准方案化疗2个疗程后与治疗前比较,ALL组和AML组血清、CSF中SDF-1α水平均明显降低(P<0.01).AL患者经标准方案化疗2个疗程后未缓解(NR)组血清、CSF中SDF-1α浓度明显高于完全缓解(CR)组(P<0.01).经标准化疗方案治疗2个疗程后,CNSL组血清、CSF中SDF-1α浓度明显高于N-CNSL组(P<0.01).结论　 SDF-1α可作为AL特别是CNSL患者的病情检测指标,有助于判断病情发展、估计预后,其表达与AL的分型可能具有一定相关性.%Objective To study the level and clinical significance of stxomal cell derivative factors 1α ( SDF-1α )in the serum and cerebrospinal fluid( CSF )of patients with acute leukemia( AL ). Methods The levels of SDF-1α in serum and CSF of patients with acute leukemia were detected by enzyme linked immunosorbent assay ( ELISA ) in 56 patients with acute leukemia who were newly diagnosed and treated according to standard chemotherapy scheme for 2 treatment courses,and 10 patients with craniocerebral trauma were enrolled as control group. Results During preliminary diagnosis without treatment, the levels of SDF-lα in serum and CSF in ALL group, AML group, CNSL group and N-CNSL group were significantly higher than those in control group( P < 0.01 ),furthermore,which in ALL group were significantly higher than those in AML group( P <0.01 ),which in CNSL group were significantly higher than those in N-CNSL group( P < 0. 01 ). After 2-month standard scheme chemotherapy, the levels of SDF-1α in serum and CSF in ALL group and AML group were significantly lower than those before treatment( P < 0. 01 ), For AL patients, After 2-month standard scheme chemotherapy the levels of SDF-1α in serum and CSF in NR group were significantly higher than those in CR group( P < 0. 01 ). After 2-month standard scheme chemotherapy,the levels of SDF-lα in serum and CSF in CNSL group were significantly higher than those of N-CNSL group( P <0. 01 ). Conclusion SDF-1α can be used as the detection indexes for patients with AL, especially for CNSL patients, which is helpful to evaluate the progress and prognosis of patient' s condition,furthermore,the expression of SDF-1α may be related with the typing of AL.