化痰祛浊通络方对自发性高血压大鼠血压一氧化氮、内皮素1及血管紧张素Ⅱ的影响

摘要

Objective To observe the effects of Huatan-quzhuo-quzhuo decoction on blood pressure, ni-tric oxide (NO), endothelin-1 (ET-1) and angiotensin-Ⅱ(AngⅡ) in spontaneously hypertensive rats (SHR), and to investigate the antihypertensive mechanism.Methods 15 -months -old rats were randomly divided into blank control group,Huatan-quzhuo-tongluo decoction group and indapamide group, 9 rats in each group.Rats in blank control group received 0.9%sodium chloride (10 mg/kg) .Rats in Huatan-quzhuo-tongluo decoction group received Huatan-quzhuo-quzhuo decoction (10 mg/kg).Rats in indapamide group received indapamide solution (10 mg/kg) , and all mice received successive perfusion once daily for 6 weeks.The arterial pressure was measured in all mice weekly, and the contents of serum NO, ET-1 and AngⅡwere detected by abdominal aorta blood-col-lecting 6 weeks after successive perfusion .Results The systolic pressure ( SP) and diastolic pressure ( DP) were decreased six weeks after successive perfusion in Huatan-quzhuo-tongluo decoction and indapamide groups, and the decreased in two groups was significantly different from that in blank control group (P<0.05).The contents of ET-1 and Ang-Ⅱafter perfusion were obviously decreased in Huatan-quzhuo-tongluo decoction and indapamide groups, and the content of NO was obviously increased, the differences were statistically significant (P<0.05).The contents of ET-1 and Ang-Ⅱafter perfusion were obviously decreased in Huatan-quzhuo-tongluo decoction and indapamide groups as compared with those in blank control group, and the content of NO was obviously increased, the differences were statistically significant (P<0.05).Conclusion Huatan-quzhuo-tongluo decoction can signifi-cantly reduce the blood pressure in SHR rats, and the possible mechanism is to decrease serum levels of ET, AngⅡand increase NO levels.%目的：观察化痰祛浊通络方对自发性高血压大鼠（SHR）血压及一氧化氮（NO）、内皮素1（ET－1）及血管紧张素Ⅱ（AngⅡ）的影响，探讨其降压作用机制。方法将15月龄SHR随机分为空白对照组、化痰祛浊通络方组和吲达帕胺组，每组各9只。空白对照组予0．9％氯化钠注射液（10 mL／kg）、化痰祛浊通络方组予化痰祛浊通络方水煎液（生药10 mL／kg）、吲达帕胺组予吲达帕胺溶液（10 mg／kg），均每日1次灌服，连续6周。所有大鼠每周测量尾动脉压，给药6周腹主动脉抽血测定血清NO、ET－1及AngⅡ含量。结果给药6周后，化痰祛浊通络方组和吲达帕胺组给药后均可降低SHR收缩压（ SP）和舒张压（ DP），与空白对照组及本组给药前比较差异均有统计学意义（P＜0．05）；化痰祛浊通络方组及吲达帕胺组ET－1和AngⅡ含量均较本组给药前及空白对照组明显降低，NO含量明显升高，差异均有统计学意义（P＜0．05）。结论化痰祛浊通络方能明显降低SHR血压，其机制可能是通过下调ET、Ang Ⅱ含量及上调NO含量来发挥降压作用。