不同产地山药中总多糖及尿囊素的含量分析

摘要

Objective To compare the quality of the Dioscorea opposite Thunb. from Henan and Hebei by determining the content of polysaccharide and allantoin. Methods Phenol-sulfuric acid method was used to determine the content of polysaccharide. Allantoin content was determined by high performance liquid chromatography. SPOLAR C18 (250mm×4. 6mm,5μm) was used. Mobile phase was acetonitrile-water (2:98). The flow rate was 0. 5 mL/ min. The column temperature was set at 25℃ . The detection wavelength was set at 224 nm. Results The polysaccharide content(% ) in the Dioscorea opposite Thunb. from Henan was (14. 65±1. 43), while the Hebei was (7. 78 ±2. 83). There was significant statistical difference between the two varieties(P<0. 01). There was no significant difference of the allantoin content between the varieties(P>0. 05). Conclusion The Dioscorea opposite Thunb. from Henan contains higher polysaccharide, which might have higher medicinal value and better quality, deserves paying close attention as a good variety.%目的：对比河南产地和河北产地山药的品质,寻找优良品种。方法总多糖含量采用苯酚硫酸法测定；尿囊素含量采用高效液相色谱法测定,色谱条件：色谱柱：资生堂 SPOLAR C18(4.6 mm×250 mm,5μm),流动相：乙腈-水(2：98),流速：0.5 mL/ min,柱温25℃,检测波长：224 nm。结果总多糖含量(%)河南产地山药中为(14.65±1.43)%,河北产地山药中为(7.78±2.83)%,二者有显著性差异(P<0.01)；在尿囊素含量上二者没有显著性差异(P>0.05)。结论河南产地的山药中含有较高的总多糖,可能药用价值更高,品质更优,是值得关注的优良品种。