樱桃谷种鸭鸭1型甲肝病毒的分离鉴定及其 VP1基因的序列分析

摘要

A virus strain,named HeN1403,was isolated from the liver of dead Cherry Valley Breeding ducks .The virus killed 10 days Muscovy duck embryo within 79 hours and showed 33.3% of mortality rate to eight-day-old Muscovy ducklings.HeN1403 was characterized by specific RT-PCR primers which target to duck hepatitis virus A type 1,and it turned out to be a 200 bp fragment.A 714-bp fragment gene was obtained by using a set of primers aimed at VP1 ORF gene.Genetic analysis showed that the isolated virus was one of pancreatitis-type duck hepatitis virus A type 1.%从某樱桃谷种鸭场病死樱桃谷种鸭的肝脏中分离到1株病毒（命名为 HeN1403），该病毒能在79 h 内致死10日龄番鸭胚，对8日龄雏番鸭的致死率为33．3％。应用鸭1型甲肝病毒特异性引物对 HeN1403分离株进行 RT-PCR 检测，可扩增到约200 bp 的目的条带，并对分离株的 VP1基因进行扩增，得到714 bp 的基因片段。通过软件对分离株 VP1基因序列与 GenBank 上发表的12株不同基因型鸭1型甲肝病毒的 VP1基因核苷酸序列的同源性进行比对和遗传进化分析，确定该分离株为鸭1型甲肝病毒。