黄豆脲酶的提取及其活性测定

摘要

对市售黄豆进行了脲酶提取研究，通过pH增值法对脲酶活性进行测定，并确定黄豆脲酶的最佳提取方案。结果表明，提取黄豆脲酶的最佳方案为：采用干黄豆为原料，以30%乙醇溶液提取脲酶粗酶液，用分级沉淀的方法对粗酶液里的脲酶进行分离，采用Sephadex G-150凝胶层析过滤装置对脲酶液进行纯化，用真空冷冻干燥技术对脲酶液进行干燥浓缩，得到酶活力最高的脲酶。%　　Method of extraction and activity determination for the urease from soybean was investigated and the best method to gain highest activity urease was proposed as follows:dry soybean was extracted by 30%alcohol. The urease solution was separated by Sephadex G-150 column chromatography and concentrated by vacuum freezing dry.