以野生苦菜为原料，采用1，1-二苯基-2-三硝基苯肼（DPPH）清除能力、2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）清除能力、羟基自由基清除能力和铁离子还原能力法评定其抗氧化能力，采用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）法评定苦菜提取物对雌激素依赖性人类乳腺癌细胞MCF-7和雌激素非依赖性人乳腺癌细胞MDA-MB-231的抑制活性。结果表明，苦菜甲醇提取物对ABTS、DPPH都具有较强的清除能力，且具有较强的铁离子还原能力，对雌激素依赖性人类乳腺癌细胞MCF-7和雌激素非依赖性人乳腺癌细胞MDA-MB-231均有显著的抑制活性。苦菜的甲醇提取物对MCF-7和MDA-MB-231半抑制率分别为99、107μg/mL。%The wild Ixeris Sonchifolia (from Shanxi, China) was used to evaluate its antioxidant activity and tumor inhibiting activity. The antioxidant activity was evaluated using the 2, 2-diphenyl-1-picrylhydracyl (DPPH·), hydroxyl free radical (OH·) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonicacid) (ABTS·+) scavenging methods and the ferric reducing antioxidant power assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to evaluate its tumor inhibiting activity. The results showed that the Ixeris Sonchifolia extracts had excellent DPPH·, ABTS·+scavenging capacity and reducing power. The Ixeris Sonchifolia extracts can significantly inhibit human breast tumor MDA-MB231 (estrogen-independent tumor) and MCF-7 (estrogen-dependent tumor). The IC50 (half maximal inhibitory concentration) of MDA-MB231 and MCF-7 were 99 and 107μg/mL, respectively.
展开▼
