石榴皮总多酚的微波辅助提取工艺及抗氧化活性研究

摘要

Response surface method was used to optimize the technological conditions of microwave assisted ex-tracting total polyphenols from pomegranate rind. Extraction yield of polyphenols as an indicator, single factor experiment and response surface method were used to discuss the influence on extraction yield by the ethanol concentration, extraction time, the microwave power and liquid-solid ratio. The regression equation prediction model was developed by Box-Benhnken center combination design and response surface method. The optimal extract conditions were as follows: ethanol concentration 58 %,extracting time 98 s, the microwave power 235 W, liquid-solid ratio 49∶1(mL/g). Under the condition, the extraction yield of polyphenols was(23.26± 0.15)%. The obtained polyphenols was characterized by IR spectra. The IC 50 values of the obtained extracts a-gainst DPPH free radicals was 0.28 mg/mL, suggesting that polyphenols from pomegranate rind had a remark-able antioxidant effect.%利用响应面分析法优化石榴皮总多酚的微波辅助提取工艺，以总多酚得率为指标，通过单因素试验及响应面分析，研究了乙醇体积分数、提取时间、提取功率和液固比4个主要因素对石榴皮总多酚得率的影响。采用Box-Benhnken中心组合设计和响应面分析法，建立了回归方程的预测模型，确定最佳提取条件为乙醇体积分数58%、提取时间98 s、提取功率235 W、液固比49∶1（mL/g）。此条件下总多酚得率为（23.26±0.15）%。试验结果与模型预测值基本相符。用红外光谱对总多酚提取物进行了表征。石榴皮总多酚对DPPH自由基的半清除质量浓度为0.28 mg/mL，具有较强的抗氧化作用。