高效液相色谱法测定淫羊藿药材及饮片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量

摘要

目的:采用高效液相色谱法,建立测定淫羊藿药材及饮片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷含量的方法.方法:采用Agilent ZORBAX SB-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.01％磷酸水(V:V=24:76),流速为1.0 ml/min,柱温为30℃,检测波长为270 nm.结果:朝藿定A、朝藿定B、朝藿定C和淫羊藿苷分别在0.068～0.408、0.316～1.896、0.280～2.240和0.434～2.604μg范围内线性关系良好,其平均加样回收率分别为103.49％、98.80％、96.75％和102.10％.结论:该方法快捷、准确,能够更好地实现对淫羊藿药材和饮片的质量控制.%OBJECTIVE: To establish the method for simultaneous determination of epimedins A , B and C and icariin in epimedii folium medicinal materials and decoction pieces by HPLC .METHODS:The samples were separated on an Agilent Zorbax SB-C18 (4.6 mm ×250 mm, 5 μm) column, with mobile phase of 0.01％ acetonitrile with phosphoric acid water ( V:V =24:76 ); the flow rate was 1.0 ml/min; the column temperature was 30 ℃; the wavelength was 270 nm.RESULTS:The linear ranges for epimedins A , B and C and icariin were respectively 0.068-0.408, 0.316-1.896, 0.280-2.240 and 0.434-2.604 μg, with average average recoveries of 103.49％, 98.80％, 96.75％and 102.10％.CONCLUSIONS:The established HPLC quantitative method is quick and accurate , and can be used to control the quality of epimedii folium medicinal materials and decoction pieces .