maFGF对慢性衰老大鼠肝组织ATP酶、SOD活力及丙二醛和羟自由基水平的影响

摘要

目的 探讨改构型酸性成纤维细胞生长因子(maFGF)对衰老大鼠肝组织中ATP酶、超氧化物歧化酶(SOD)活力及丙二醛(MDA)、羟自由基(OH·)水平的影响.方法 采用皮下注射D-半乳糖建立衰老大鼠模型,将其随机分为衰老模型组、生理盐水对照组和maFGF治疗组.另10只不给任何药物,正常饲养,作为正常对照组.测定各组肝组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力以及MDA、OH·水平.结果 衰老模型组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力均显著降低,MDA和OH·基含量均升高(P＜0.05);maFGF治疗组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力均显著升高,MDA和OH·含量均降低(P＜0.05).结论 maFGF通过降低自由基,提高ATP酶活力和抗氧化能力而对衰老大鼠有保护作用.%Objective To study the effects of modified acidic fibroblast growth factor(maFGF) on activities of ATPase and su-peroxide dismutase(SOD) ,levels of malondialdehyde(MDA) and hydroxyl radical(OH · ) in liver tissue of aging rats. Methods Aging rat models were established by subcutaneous injection of D-galactose. They were randomly divided into aging model group, physiological saline control group and maFGF treatment group. Other 10 rats accepted drug-free normal feeding served as normal control group. Activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and SOD, levels of MDA and OH · In liver tissue were measured. Results In aging model group, activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase and SOD in liver tissue of rats were markedly decreased, while levels of MDA and OH · Increased(P<0. 05). In maFGF treatment group, activities of Na+-K + -AT-Pase,Ca2 -Mg2 -ATPase and SOD in liver tissue of rats were significantly increased, while levels of MDA and OH · Decreased (P<0. 05). Conclusion maFGF exhibit a protective effect on aging rats via reducing free radicals,enhancing ATPase activities and antioxidant capacity.