飞机草镇痛作用部位及其机制研究

摘要

目的：明确飞机草镇痛作用部位及其可能的作用机制，为充分合理利用飞机草资源提供参考。方法将小鼠分成6组，采用脊髓化小鼠甩尾法，福尔马林致炎性疼痛法及纳洛酮阻断小鼠扭体法、热板法，分析飞机草镇痛作用部位；应用酶联免疫法检测血清和脑组织中NO和前列腺素E2（PGE2）含量，初步研究其镇痛作用机制。结果实验结果表明高剂量的飞机草能明显提高脊髓化小鼠热刺激甩尾反应痛阈值（P＜0．05）；高、中剂量的飞机草对福尔马林致痛早期相、晚期相均有显著镇痛作用（P＜0．05），明显减少纳洛酮阻断后冰醋酸性腹膜炎症致痛小鼠扭体次数，并明显延长纳洛酮阻断后热板致痛小鼠舔后足时间（P＜0．05），能显著降低福尔马林致炎疼痛小鼠脑组织和血清中NO和PGE2含量（P＜0．05）。结论飞机草既有中枢也有外周的镇痛作用，这可能与其降低中枢和外周NO和PGE2含量有关。%Objective To study the analgesic effect of Eupatorium odoratum L and its mechanism .Methods Mice were random‐ly divided into 6 groups .Swinging tail method in spinal cord destroyed mouse ,hot‐plate and twisted body method in naloxone blocked mouse ,and formalin‐induce inflammatory pain method were done to investigate the analgesic effect of Eupatorium odoratum L .Each pain index under each experiment condition was determined after intra gastric administration of each group of mice .The concentration of NO and PGE2 in serum and brain were measured to study its mechanisms .Results High‐dose of Eupatorium odor‐atum L significantly enhanced pain threshold in either spinal cord destroyed or naloxone blocked mouse with pain induced by hot and acetic acid(P<0 .05) .High and middle dosage groups of Eupatorium odoratum L markedly reduced the first‐and second‐phase re‐sponses of formalin‐induced inflammatory pain mouse(P< 0 .05) ,and decreased the writhing times and licking rear feet times caused by twisted body method(P<0 .05) .The level of NO and PGE2 in serum and brain of mouse induced by formalin were both decreased(P<0 .05) .Conclusion Eupatorium odoratum L has notable analgesic effect ,which may be related to reduce the level of NO and PGE2 in central and peripheral site .