一对检测肺炎衣原体的新引物

摘要

Aim In order to improve the detection and identification ofChlamydia pneumoniae,a new primer pair(53A,53B)for PCR were designed based on the species-specific 53kDa protein gene of C.pneumoniae.The primers permitted the identification of 2 C.pneumoniae strains-AR-39 and VR1310,but no reaction was observed with C.trachomatis TE-55 and L2/434/Bu strains nor C.psittaci B11001 strain.The primers were also unable to amplify the DNA of bacteria commonly related to respiratory tract in fections.The positive amplification primers was achieved with only 10fg DNA of C.pneumoniae,which was more sensitive than the previous primers Cpn1,Cpn2.Therefore,the new primers will be use in the diagnosis of C.pneumoniae infections.%为建立一种更敏感的肺炎衣原体PCR检测方法，根据肺炎衣原体种特异性53kDa蛋白基因序列设计一对引物53A、53B，用PCR进行肺炎衣原体的检测研究。结果该引物仅能扩增肺炎衣原体DNA，而与沙眼衣原体，鹦鹉热衣原体不反应，且对呼吸道常见病原菌之DNA的扩增亦为阴性，它能扩增出少至10fg的肺炎衣原体DNA，比我们以前合成的一对肺炎衣原体16srRNA基因检测引物Cpnl、Cpn2更敏感，分别用这两对引物检测30例有呼吸道疾患病人的鼻咽拭子标本，PCR的阳性符合率为86.7%。表明53A、53B是一对有效、实用、敏感的肺炎衣原体检测引物。