羊口疮病毒F1L蛋白二级结构分析与表位预测

摘要

The network platform SOPM A server was used to predict the secondary structure of ORFV F 1L protein . On the basis of the DNAStar prediction results (hydrophilicity , accessibility , flexibility and antigenicity ) , the B cell epitopes of ORFV F1L protein was predicted by screening the secondary structure and followed by verification using ABC prod program . The cytotoxic T cell (CTL) epitopes of ORFV F1L protein were predicted by combination of quantitative matrix (QM ) and artificial neural network (ANN) . T helper (Th) epitopes of ORFV F1L protein were predicted by an online server for the prediction of M HC class II-peptide binding affinity . The result showed that ORFV F1L protein had alpha helixes (34 .71% ) , random coils (40.59% ), β-sheet (18.82% ) and β-turn (5.88% ). No signal peptide was found, while 7 B-cell epitopes, 2 CTL epitopes and 3 Th epitopes of ORFV F1L protein were predicted . These findings would provide theoretical basis for establishment of ORFV diagnosis method , preparation of monoclonal antibody and development of vaccine .%目的 利用软件预测羊口疮病毒(ORFV)F1L蛋白的二级结构和细胞表位.方法 利用SOPMA服务器预测ORFV F1L蛋白的二级结构,以DNAStar软件单参数(二级结构、亲水性、可及性、柔韧性及抗原性)预测结果为基础,通过二级结构预测初步筛选,并以ABCpred方案作为最终验证,预测羊口疮病毒(ORFV) F1L蛋白的B细胞表位;运用神经网络+量化矩阵法(ANNs+QM法)预测ORFV F1L蛋白的CTL细胞表位;使用MHC-II类分子结合肽在线程序预测ORFV F1L蛋白的Th细胞表位.结果 ORFV F1L蛋白含有α-螺旋34.71%、β-片层18.82%、β-转角5.88%、无规则卷曲40.59%;没有信号肽,可能存在7个B细胞表位, 2个CTL表位,3个Th细胞表位.结论 该研究结果将为建立ORFV诊断方法、制备单克隆抗体及合成肽疫苗提供理论依据.