二氢槲皮素与二氢杨梅素抗炎活性对比研究

摘要

Construction of macrophages to validate the model,comparative study dihydroquercetin(also known as taxifolin,TF)and dihydromyricetin (DMY)in vitro anti -inflammatory activity was made.To 1 mg/L lip polysaccharide (LPS) -induced RAW264.7 macrophages in vitro model of inflammation was established;Cell viability was detected by the cytotoxicity test (MTT)method;Griess reagent method was used to determine the amount of NO released from cell supernatant;The content of cytokine IL -1β,IL -6,TNF -αand PGE2 in supernatant was detected by ELISA assay;Gene expression were detected with the methods of RT -PCR.TF and DMY 2.5 ~20 μg/mL showed no cytotoxicity on macrophages of RAW264.7.Meanwhile,TF high,medium and low dose group and DMY high,medium and low dose group significantly inhibited LPS -induced cells to release of NO and PGE2(P <0.01),and showed a dose -dependent manner;TF high,medium and low dose group and DMY high,medium and low dose group could effectively reduce IL -1β,IL -6,TNF -αgene expression,while reducing LPS -induced cells to produce cytokines levels.Dihydroquercetin and DMY have good anti -inflammatory effects in vitro and anti -inflammatory effect of TF was better.%构建巨噬细胞验证模型，比较研究二氢槲皮素（Dihydroquercetin，taxifolin，TF）和二氢杨梅素（Dihydromyricetin，DMY）体外抗炎活性。以1 mg／L 的脂多糖（LPS）诱导 RAW264．7巨噬细胞建立了体外炎症模型；通过细胞毒性试验（MTT）法检测细胞存活率；Griess 试剂法测定细胞上清液中 NO（一氧化氮）释放量；ELISA 法检测上清液中细胞因子 IL －1β（白细胞介素－1β）、IL －6（白细胞介素－6）、TNF －α（肿瘤坏死因子α）和 PGE2（前列腺素 E2）的含量变化；通过 RT －PCR 法检测IL －1β、IL －6、TNF －α基因表达情况。MTT 试验结果表明，二氢槲皮素和二氢杨梅素各剂量组没有表现出对巨噬细胞 RAW264．7的细胞毒性。同时，二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能显著抑制脂多糖诱导细胞对 NO、PGE2的释放（P ＜0．01），且均表现出剂量依赖性；二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能有效降低 IL －1β、IL －6、TNF －α基因的表达，同时能够降低脂多糖诱导细胞产生细胞因子 IL －1β、IL －6、TNF －α的含量。二氢槲皮素和二氢杨梅素均具有良好的体外抗炎作用，且二氢槲皮素的抗炎作用较好。