电刺激大鼠上矢状窦旁硬脑膜后血浆降钙素基因相关肽和P物质的变化

摘要

Objective To analyze the changes of plasma concentration of calcitonin gene-related peptide (CGRP) and substance P (SP) after electrical stimulation on dura mater adjacent to sinus sagittalis superior (SSS) in rats. Methods 13 rats were randomly divided into control group(n= 6) and stimulation group(n= 7) in which the dura matter adjacent to SSS was stimulated electrically with square wave of 20 Hz, 4 mA and 250 μs. The plasma concentrations of CGRP and SP in both groups were compared by means of radioimmunity method. Results There was significant difference in the plasma concentration of CGRP between control and stimulation groups (50. 60±7. 16 pg/ml vs 42. 20±4.82 pg/ml, P=0.029). However, there was no significant difference in the plasma concentration of SP between two groups (308. 59±43. 07 pg/ml vs 264. 60±60.28 pg/ml, P=0. 165). Conclusion Plasma concentration of CGRP decreased after electrical stimulation on dura mater in rats but the concentrations of SP unchanged.%目的观察电刺激上矢状窦旁硬脑膜对大鼠血浆降钙素基因相关肽(CGRP)和P物质浓度的影响.方法 13只大鼠随机分为对照组(n=6)和电刺激组(n=7).对照组行假刺激,电刺激组以20 Hz、4 mA和250μs的方波电流刺激上矢状窦旁硬脑膜,使用放射免疫法比较血浆CGRP和P物质的浓度.结果对照组与电刺激组的血浆CGRP浓度相比有显著性差异(50.60±7.16 pg/ml vs 42.20±4.82 pg/ml,P=0.029),而血浆P物质浓度相比无显著性差异(308.59±43.07 pg/ml vs 264.60±60.28pg/ml,P=0.165).结论电刺激上矢状窦旁硬脑膜能够降低大鼠血浆CGRP浓度,而P物质浓度无明显变化.