尼古丁戒断效应对大鼠种植早期骨愈合的影响

摘要

Objective: To study the nicotine dependence-abstinence effect on the early osseointagration and the expression of the OPG and BMP-2 after implantation. Methods: Thirty-six SD rats were equally randomly applied for control group, Nicotine-De pendence group (ND), and Nicotine Dependence-ab-stinence group(NW) and subcutaneous injection with 0.1ml/tid saline, 3mg/kg/tid nicotine and 3mg/kg/tid nicotine respectively for 7 days, subcutaneous injected 1mg/kg mecamylamine after 1 hour at the end of injection, and observe the nicotine abstinence symptoms. Then the two tibias of the rats were implanted by the titanium implant with the surface acid-sandblasting process. NW group stop nicotine intervention, Control group and ND group continue to exert interventions. The new bone generation, the bone mineralization, the expression of BMP-2 and OPG, the bone reconstruction of the specimens were observed by X-ray, CT, RT-PCR and SEM (scanning electron microscope) at the end of 7th and 14th days after implantation. Result: Compared with control group, the new bone generation and the expres-sion of BMP-2 of ND group and NW group was no significant difference, while the bone mineralization, the expression of OPG and the bone reconstruction of ND group was reduced, and the NW group de-creased more significantly. Conclusion: Nicotine dependence will hinder the early osseointagration sur-rounding implant and the expression of BMP-2 and OPG, and the nicotine abstinence effects can not ease this effect, they both influence the early stability of the implant.%目的：研究戒断效应对尼古丁依赖的大鼠早期种植后骨愈合及BMP-2和OPG表达情况的影响。方法：36只SD大鼠随机数表法分为对照组（Control group）、尼古丁依赖组（Nicotine-dependence group， ND）、尼古丁依赖-戒断组（nicotine-withdrawal group， NW），每组12只，分别每次皮下注射0.1ml生理盐水、3mg/kg尼古丁、3mg/kg尼古丁，每天3次，连续7天，最末次注射1h后皮下注射1mg/kg美加明，并观察尼古丁戒断症状。尔后大鼠左右胫骨近干骺端均植入表面经喷砂酸蚀处理的种植体， NW组停用尼古丁干预，对照组和ND组继续施加干预措施。种植术后第7、14天通过X线和CT观察大鼠种植体周围新骨生成量和骨矿化程度， SEM观察种植体表面骨改建情况， RT-PCR测量BMP-2和OPG的表达量。结果：种植术后7、14天数据显示，尼古丁依赖及其戒断效应对种植体周围新骨生成量无明显影响，但观察种植体周围骨密度值、扫描电镜和骨活性基因BMP-2、OPG表达时发现， ND组与NW组种植体周围骨组织矿化、改建的程度和基因的表达量均低于对照组。结论：尼古丁依赖会阻碍种植体周围的骨愈合和BMP-2、 OPG的表达，而早期的尼古丁戒断效应不会缓解这种阻碍作用，尼古丁的依赖与戒断效应均影响种植体的早期稳定。