Objective: To observe the effects of ShenShuNing on the peritoneal function, peritoneal matrix accumulation, neovascularization , the expressions of Connective tissue growth factor( CTGF ), Vascular endothelial growth factor( VEGF ) Hepatocyte growth factor( HGF )、Bone morphogenetic protein,( BMP -7 ) in the peritoneal fibrosis correlating peritoneal dialysis rats. Methods: peritoneal fibrosis correlating peritoneal dialysis SD rat model was induced by injecting adriamycin and peritoneal dialysate. They were randomly divided into the model group,the 1.5% peritoneal dialysate group ,the 4. 25% peritoneal dialysate group,the 1.5% peritoneal dialysate and ShenShuNing group and the 4. 25% peritoneal dialysate and ShenShuNing group according to body weight. Besides, another 15 rats was taken as the blank control group. Of them, ShenShuNing ( 43. 93 g · kg-1· d-1 ) was given to rats in theperitone-al dialysate and ShenShuNing groups by gastrogavage, while the same normal saline was given to rats in other groups by gastrogavage. The changes of glucose in peritoneal fluid , Ultra Filtration volume( UF ), Mass transfer of glucose( MTG )were detected. The patho-morphological changes of the peritoneum were observed. Peritoneal thickness, vessel count, Fibronectin( FN ), expressions of CTGF, VEGF, HGF and BMP - 7 were determined. Results: At the end of the 6th week, significant difference was shown in peritoneal dialysate and ShenShuNing groups UF( - 3. 26 ± 14. 17 )ml and ( - 2. 04 ± 10. 74 )ml, MTG ( 18. 12 ± 0. 81 )mmol/kg and ( 16. 14 ± 1.20 ) mmol/kg,peritoneal thickness ( 74.68 ±15.33 )μm and ( 211.75 ±58.23 )μm,vessel count ( 8.21 ±2.66 ) mm2 and ( 13.70 ± 4.71 ) mm2,FN( 152.47 ±36. 94 )% and (( 231.49 ±53.49 )%,CTGF ( 708.67 ± 127. 22 )% and ( 791. 20 ± 126. 37 )%,VEGF (0.42 ±0.08)% and (0.50 ±0.09)% ,HGF( 245.71 ±24.38)% and (222. 05 ±23.43 )%,BMP-7 ( 351. 27 ± 31. 99 )% and ( 284.43 ±26.82 )% , when compared with the same peritoneal dialysate groups ( P < 0. 05, P < 0. 01 ). Conclusion: Shenshuning could postpone the development of peritoneal fihrosis in peritoneal fibrosis correlating peritoneal dialysis SD rat model possibly through regulating the expressions of CTGF, VEGF, HGF and BMP - 7 , hindering the over - accumulation of peritoneal matrix and neovascular-ization.%目的:观察中药肾疏宁对腹膜纤维化大鼠腹膜功能、腹膜组织基质积聚、新生血管、结缔组织生长因子(CTGF)、血管内皮生长因子(VEGF)、肝细胞生长因子(HGF)及骨形态蛋白-7(BMP-7)表达的影响.方法:腹膜透析液+阿霉素注射法诱发SD大鼠腹膜透析腹膜纤维化模型,依体重随机分为4组,1.5%腹膜透析液模型组、4.25%腹膜透析液模型组、1.5%腹膜透析液+肾疏宁干预组及4.25%腹膜透析液+肾疏宁干预组,每组各15只,另设空白对照组(对照组,15只).1.5%腹膜透析液+肾疏宁干预组及4.25%腹膜透析液+肾疏宁干预组予肾疏宁(按43.93 g·kg-1·d-1)灌胃,对照组和模型组予等量生理盐水灌胃.检测腹透液葡萄糖浓度,计算超滤量(UF)及葡萄糖转运量(MGT).观察腹膜病理形态学改变,分析腹膜厚度、腹膜血管计数、腹膜纤维连接蛋白(FN)及CTGF、VEGF、HGF、BMP-7表达.结果:治疗第6周末,1.5%腹膜透析液+肾疏宁干预组及4.25%腹膜透析液+肾疏宁干预组腹膜透析腹膜纤维化大鼠超滤量(-3.26±14.17) ml及(-2.04±10.74) ml、葡萄糖转运量(18.12±0.81) mmol/kg及(16.14±1.20) mmol/kg、腹膜厚度(74.68±15.33) μm及(211.75±58.23) μm、腹膜血管计数(8.21±2.66)个/mm2及(13.70±4.71)个/mm2、FN(152.47±36.94)%及(231.49±53.49)%、CTGF(708.67±127.22)%及(791.20±126.37) %、VEGF(0.42±0.08)%及(0.50±0.09)%、HGF(245.71±24.38)%及(222.05±23.43)%、BMP-7(351.27±31.99)%及(284.43±26.82)%,与同浓度透析液组比较,差异有统计学意义(P＜0.05,P＜0.01).结论:肾疏宁可能通过调控CTGF、VEGF、HGF、BMP-7表达,阻抑基质过度积聚及新生血管生成,进而延缓腹膜透析大鼠腹膜纤维化进展.
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