目的 观察APP/PS1转基因小鼠胶质纤维酸性蛋白(GFAP)在海马结构3个亚区星形胶质细胞内的表达变化,同时对比观察亚甲蓝对其表达改变的影响.方法 20只3月龄APP/PS1小鼠,随机分2组,每组10只,对照组:自由饮水；治疗组:根据小鼠饮水量将亚甲蓝加入日常饮水中(25 mg·kg-1 ·d-1)连用4个月至7个月龄.结果 免疫组织化学结果表明治疗组APP/PS1转基因小鼠海马结构3个亚区星形胶质细胞GFAP的表达与对照组相比下调(P＜0.05).Western印迹检测结果显示,治疗组小鼠海马结构3个亚区星形胶质细胞GFAP表达明显弱于对照组(P＜0.05).结论 亚甲蓝可以下调APP/PS1转基因小鼠海马结构3个亚区星形胶质细胞GFAP的表达.%Objective To examine the expression of glial fibrillary acidic protein (GFAP) in hippocampal formation of astrocyte of APP/PS1 double-transgenic mice and effects of methylene blue (MB) on it. Methods 20 APP/PS1 transgenic mice were assigned into two groups. Mice in control groups were received normal drinking water while in treated groups were received drinking water mixed with MB (25 mg·kg-1·d-1). Treatment was started when the mice were 3 months old and continued for 4 months. Results Immunohistochemical staining and western blotting showed that the expression of GFAP in hippocampal formation of the mice in MB treated group was decreased (P < 0. 05 ). Conclusions MB may down-regulate the expression of GFAP in hippocampal formation of APP/PS1 double-transgenic mice.
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