全自动固相萃取-气相色谱-串联质谱法测定卷烟主流烟气中的3种多环芳烃

摘要

A simple method for the analysis of benzo [ a ] pyrene, benzo [ a ] anthracene and chrysene in mainstream cigarette smoke using automated solid phase extraction (SPE) system-gas chromatography-tandem mass spectrometry ( GC-MS/MS) was developed. After being smoked in the ISO mode, the Cambridge filter pad was used to collect mainstream cigarette smoke, and then extracted by 40 mL cyclohexane containing deuterated benzo [ a ] pyrene as the internal standard. The extract was cleaned-up by an automated PrepLinc SPE-AccuVap system, and then detected using GC-MS/MS. The detection limits for benzo [ a ] pyrene, benzo [ a ] anthracene and chrysene were 0.05, 0. 16, and 0.23 ng/cig, respectively. The recoveries were ranged from 91. 5% to 102. 1% with the relative standard deviations (RSDs) less than 5%. This method was proved to be simple, sensitive, and suitable for the determination of benzo[ a]pyrene, benzo[ a] anthracene and chrysene in mainstream cigarette smoke.%以全自动固相萃取技术净化主流烟气萃取液,建立了卷烟主流烟气中苯并[a]芘、苯并[a]蒽和(艹屈)3种多环芳烃的气相色谱-串联质谱(GC-MS/MS)测定方法.以吸烟机抽吸卷烟,并以剑桥滤片捕集卷烟主流烟气,然后以含氘代苯并[a]芘内标的环己烷溶液萃取滤片,萃取液经全自动固相萃取仪净化后以GC-MS/MS分离检测.结果表明,苯并[a]芘、苯并[a]蒽和(艹屈)的检出限分别为0.05、0.16和0.23 ng/cig,回收率为91.5％ ～ 102.1％,相对标准偏差(RSD)均小于5％.该方法的自动化程度高、操作简便、检出限低、重复性好,适用于卷烟主流烟气中苯并[a]芘、苯并[a]蒽和(艹屈)3种多环芳烃释放量的检测.