基于激光剥蚀-电感耦合等离子体质谱技术的生物元素成像分析

摘要

Trace elements play a very important role in biological organism and closely related to many diseases. The new analytical methods are urgently needed for in-situ determination of trace elements in biological tissues or single cells. A method based on laser ablation-inductively coupled plasma-mass spectrometry ( LA-ICP-MS) was developed for such in-situ analysis. Line ablation mode was chosen and the output energy of the laser was also optimized at a low fluence of less than 1 J/cm2 . The two kinds of elemental bio imaging of both a coronal section from mouse brain and single cells exposed to gold nanoparticles were obtained by the developed method. Because of the unique characteristics, such as good spatial resolution, excellent detection limit, and reasonable running cost, LA-ICP-MS will be applied widely and become a useful tool in biomedical research in the future.%生物体内的微量元素具有十分重要的生物功能，也与许多疾病密切相关。现代生物医学的研究亟需能在组织、细胞等不同水平上原位分析生物样品中微量元素的分析方法。本研究建立了激光剥蚀-电感耦合等离子体质谱( LA-ICP-MS)原位分析生物样品的方法。采用线扫描模式和较小的激光输出能量(﹤1 J/cm2)，得到了鼠脑切片和金纳米颗粒暴露后单细胞的金属元素成像图。 LA-ICP-MS具有空间分辨率高、检出限好、运行成本较低等优势，有望在生物医学研究中得到更广泛的应用，发挥更重要的作用。