以牛血清白蛋白为稳定剂和还原剂,采用一步法合成荧光金纳米簇,并对其进行了表征.制备的荧光金纳米簇呈较规则的球形,粒径均一,约为(2.00 ± 0.05) nm,在紫外灯下发出明显的红色荧光,最大激发波长和发射波长分别为360和635 nm.Hg2+可与制备的荧光金纳米簇特异性结合而使其荧光猝灭,基于此建立了"turn-off"型的荧光光谱法快速检测Hg2+含量.优化了荧光金纳米簇的用量、pH值、检测体系等条件.荧光强度与Hg2+浓度有良好的线性关系,在0.5 ~75.0 μg/L范围内的线性方程为 y=-26.76lgx + 803.1 (R2=0.9951),在75~900 μg/L 浓度范围内的线性方程为 y=-0.27x + 762.02(R2=0.9959),检出限为0.14 μg/L (3σ).在最优条件下,本方法可在3 min 内完成检测.可快速、灵敏、简便地检测自来水中的Hg2+,自来水样品加标回收率在86.8% ~113.4%之间(n=3),相对标准偏差小于15%.%One-step green synthetic approach,with bovine serum albumin(BSA) as stabilizer and reductant, was developed for preparation of BSA hybrid fluorescence gold nanoclusters (AuNCs@BSA). The prepared AuNCs@BSA exhibited strong red fluorescence under UV light illumination. Upon excited at 360 nm, the fluorescence spectrum of AuNCs@ BSA exhibited maximum emission peak at 635 nm. AuNCs@ BSA was presented as uniform spherical morphology with diameter at (2.0 ±0.05) nm. The fluorescence of AuNCs@BSA could be quenched by Hg2+because of its metallophilic reaction. Based on the fluorescent spectrometry, a rapid detection system was developed for Hg2+detection in tap water. The AuNCs@BSA amount, pH and buffer system were optimized in this study. According to optimization results, ultrapure water (pH 5.0) was selected to dilute the AuNCs@BSA by 100 times, and 50 μL/well of AuNCs@BSA dilution was applied to detect mercury ion in tap water. Under the optimized conditions, the detection could be completed within 3 min,the fluorescence intensity of the system was linearly proportional to the concentration of mercury ion in the range of 0.5–900 μg/L with linear equations y=-26.76lgx+803.1(0.5-75 μg/L,R2=0.9951) and y=-0.27x+762.02 (75-900 μg/L,R2=0.9959). The limit of detection was 0.14 μg/L(3σ). The average recoveries in spiked tape water samples ranged from 86.8%-113.4% with relative standard deviation of less than 15%. The result implied that the developed method was able to apply to detect mercury ion rapidly, sensitively and conveniently.
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