低聚木糖修饰对牛乳β-乳球蛋白功能性质的影响

摘要

β-Lactoglobulin(β-LG)was glycosylated by xylo-oligosaccharide(XOS) under condition of different mass ratio(2:1 ,4:1,6:1,8:1, 10:1). The change of its functional properties such as solubility, emulsifying property, emulsion stability and foaming ability was studied. The results showed that degree of graft(DG)of GRPs reached 28.1% which was highest when the mass ratio of XOS and β-LG was 6:1, and when the mass ratio of XOS and β-LG was 10:1, its solubility improved best. When it's 6:1, the emulsifying property improved best. It showed that the solubility and emulsifying property of XOS glycosylation product were increased by 71.4% and 40.2%. The mass ratio of XOS and β-LG had no notably effect on foaming ability.%以低聚木糖(XOS)为修饰剂,采用不同的质量比对β-乳球蛋白进行糖基化修饰,研究了糖基化前后的β-乳球蛋白功能性质的变化.结果表明,质量比为6:1的糖蛋白配比时低聚木糖和β-乳球蛋白的糖基化反应的接枝度最高,达28.1％；与未被糖基化的β-乳球蛋白相比,10∶1的低聚木糖修饰β-乳球蛋白后,溶解性最高增加了71.4％;6∶1的低聚木糖修饰后显著提高β-乳球蛋白的乳化性及其稳定性,乳化性最高增加了40.2％；而不同的糖蛋白配比对β-乳球蛋白起泡性没有显著影响.