The DNA in Daqu extracted by the SDS-enzymic technique and diagnostic method, was diluted with gradiented concentration, and finally was amplificated. The SDS-enzymic technique can extract 300 times larger amount DNA than the diagnostic method. The optimal amplification condition was that to didute 50 times, and add the BSA in a concentration of 0.6 ng/ul. It could extract plenty of total DNA by the SDS-enzymic technique, and it can amplificate the total DNA in a effective way when diluted the total DNA and add some BSA.%利用SDS-酶法、试剂盒法提取大曲总DNA,对粗提DNA梯度稀释稀释,同时按浓度梯度加入BSA进行PCR扩增.SDS-酶法提取总DNA的量是试剂盒法的300倍；对粗提DNA稀释50倍同时添加BSA浓度为0.6ng/μL的PCR扩增效果最佳.利用SDS-酶法能够最大量的提取曲药中总DNA,对总DNA进行稀释同时加入BSA能够有效的进行PCR扩增.
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