Quantification of total bacterial DNA by real-timePCR and detection of bacteria by nested PCR frompostoperative maxillary cyst

摘要

The aims of this study were to determine the bacterial density and to examine the presence of 20 bacteria in fluids of postoperative maxillary cysts (POMC). POMC fluids (4 purulent, 2 mucous and 4 serous) were sampled from 10 subjects (aged 43 to 77). Bacterial quantification and detection were performed by real-time PCR and nested PCR based on bacterial 16S rRNA genes, respectively. Bacterial DNA was detected in all samples and the average concentrations of bacterial DNA were 5.9 (purulent), 0.5 (mucous) and 0.7 (serous) ng/mg of sample. Twelve bacterial species were detected in the purulent fluids, while 2 and 5 species were detected in the mucous and serous fluids, respectively. Purulent fluids contained numerous bacteria of various types, suggesting that oral bacteria cause symptoms such as pain in POMC with purulent fluids. Mucous and serous fluids also contained bacteria, although their numbers were small.