Objective To establish an HPLC method for the determination of arbutin in fukang freckle-removing gel. Methods Arbutin was determined by HPLC with Lichrospher-C18 column( 250 mm ×4. 6 mm,5 μm )as the chromatographic column and water-methanol (90:10 )as the mobile phase . The flow rate was 1. 0 mL · min-1 and the detection wavelength was set at 283 nm. Results The linearity of the arbutin concentration and the peak area were both promising within the range of 0.055 3 ～0. 276 5 g · L-1 ( r =0. 999 9 ). The average recovery was 99. 89% with RSD of 1. 03% . Conclusion The method is simple and accurate,and can be used for the analysis of arbutin in fukang freckle-removing gel.%目的 建立高效液相色谱法方法测定肤康祛斑凝胶中熊果苷的含量.方法 采用Lichrospher-C18色谱柱(4.6 mm×250 mm,5 μm),水-甲醇(90:10)为流动相,流速:1.0 mL·min-1,检测波长:283 nm,柱温为25℃.结果 熊果苷在0.055 3~0.276 5 g·L-1的范围内呈良好的线性关系,r=0.999 9,平均回收率为99.89%,RSD为1.03%(n=9).结论 该法简便、准确,可作为该制剂的定量分析方法.
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